T7 RNA Polymerase GMPsize:grade 250 U/μL size:10 KU
This product is a bacteriophage T7 RNA polymerase derived from recombinant expression of Escherichia coli. It uses double-stranded DNA containing the T7 promoter sequence (5'-TAATACGACT CACTATAG*-3') as a template and NTP as a substrate to synthesize RNA complementary to the reverse single-stranded DNA downstream of the promoter. Double-stranded linear blunt-end or 5' protruding end DNA can be used as a substrate template for T7 RNA polymerase, so linear plasmids and PCR products can be used as templates for in vitro RNA synthesis.
Note: G* is the first base of RNA transcription
This product is produced in accordance with GMP regulations and is provided in liquid form with an enzyme activity concentration of 250 U/μL.
Product Features
- Very high yield: 1ug DNA template can produce 100-200 µg
- General: Suitable for transcription of 20nt-10000nt RNA
- High performance: Reduce by-products in the IVT process
- Detection of no endonucleases, exonucleases, RNases
If it’s genuine T7, it’s genuine for the RNA.
